Applications of Continuous and Steady-State Methods to Root Biology.pdf

Applications of Continuous and Steady-State Methods to Root Biology PDF

This book provides an excellent illustration of the interrelationship between progress in scientific methodology and conceptual advances, and its publica­ tion should contribute to further advances. It is well known that major advances in understanding often follow the development of new methods. The development of the acetylene reduction assay for nitrogenase activity provides a good example of this interrelationship between theory and methods. Theoretical knowledge led to a search for substrates for nitro­ genase that could be assayed for more easily than ammonium, the normal product of the enzyme. The discovery of the reduction of acetylene to ethylene by nitrogenase provided the ideal answer to the problem by provid­ ing a rapid, specific, nondestructive, and inexpensive assay for nitrogenase activity. This assay is now used by almost every laboratory doing research on nitrogen fixation. However, further use and development of the acetylene reduction assay has shown that it can underestimate nitrogenase activity and can even give incorrect relative values under some circumstances. The major problem is that exposure of legume nodules to acetylene can cause a large increase in the resistance to oxygen diffusion into the nodule. This reduced supply of oxygen decreases the rate of nitrogenase activity within a few minutes.

On the approximation of continuous dispersal … Methods that do not create outcomes that tend towards the continuous process that they are supposed to approximate will produce an artefact of approximation and may bias the interpretation of such results. Except at very fine scale resolutions, we have shown here that centroid‐to‐centroid and area‐to‐centroid dispersal do not exhibit

9401075026 ISBN
Englisch SPRACHE
Applications of Continuous and Steady-State Methods to Root Biology.pdf


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